Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
نویسندگان
چکیده
منابع مشابه
Single cell measurement of dopamine release with simultaneous voltage-clamp and amperometry.
After its release into the synaptic cleft, dopamine exerts its biological properties via its pre- and post-synaptic targets(1). The dopamine signal is terminated by diffusion(2-3), extracellular enzymes(4), and membrane transporters(5). The dopamine transporter, located in the peri-synaptic cleft of dopamine neurons clears the released amines through an inward dopamine flux (uptake). The dopami...
متن کاملSingle-cell Patch-clamp Measurement System
29 THE 4TH ANNUAL IEEE INTERnational Conference on Nano/Microengineered Molecular Systems (IEEE-NEMS) was held at the Sheraton Dameisha Resort in Shenzhen, China, 5–8 January 2009. More than 300 papers were presented by leading micro/nanotechnology researchers in academia and industry from around the globe. Topical areas of the conference included microelectro mechanical systems (MEMS), BioMEMS...
متن کاملVoltage-clamp measurement of visually-evoked conductances with whole-cell patch recordings in primary visual cortex.
Whole cell patch recordings have been realized in the primary visual cortex of the anesthetized and paralyzed cat, in order to better characterize input resistance and time constant of visual cortical cells in vivo. Measurements of conductance changes evoked by visual stimulation were derived from voltage clamp recordings achieved in continuous mode at two or more different subthreshold holding...
متن کاملVoltage Clamp Experiments on Single Muscle Fibers of Rana pipiens
A voltage clamp for single muscle fibers has been developed. Stability of the system was achieved when an artificial node was created by enclosing a single muscle fiber in a petroleum jelly seal which served as an analogue of the myelin sheath. Typical voltage clamp records were obtained with large inward transient currents followed by a delayed rectification of the outward currents. These curr...
متن کاملErrors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings
Patch-clamp recording techniques have revolutionized understanding of the function and sub-cellular location of ion channels in excitable cells. The cell-attached patch-clamp configuration represents the method of choice to describe the endogenous properties of voltage-activated ion channels in the axonal, somatic and dendritic membrane of neurons, without disturbance of the intracellular milie...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2012
ISSN: 1940-087X
DOI: 10.3791/3798